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Check it out, here's every appearance Harley has made throughout comics and cartoons! I didn't keep track in my absence so this list has been reproduced with kind permission by the unbelievably cool Jeremy from Collecting Harley - a fabulous site!

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HARLEY'S MAINSTREAM COMIC APPEARANCES

 
 Batman Chronicles Gallery #1
(1996) [Note: Pin-Up]
 Batman: No Man's Land Gallery #1
(May, 1999) [Note: Pin-Up]
 Batman: Harley Quinn #1 (August, 1999) [Note: First Canon Appearence]
 Batman #570
(August, 1999): "The Code, Part One: Breakin' the Law"
 Detective Comics #737 (August, 1999): "The Code, Part Two"
 Batman: No Man's Land Secret Files #1
(October, 1999) [Note: Profile Appearance Only]
 Batman: Shadow of the Bat #93 (November, 1999): "Assembly Redux"
 Batman #573
(November, 1999): "Shellgame, Part One: Gambits"
 Azrael: Agent of the Bat #60 (November, 1999): "Evacuation"
 Detective Comics #740
(December, 1999): "Shellgame, Part 2"
 Batman: Legends of the Dark Knight #126 (December, 1999): "Endgame, Part One: Silent Night, All is Calm, All is Bright..."
 Batman #574
(December, 1999): "Endgame, Part Two"
 Detective Comics #741 (December, 1999): "Endgame, Part Three: Sleep in Heavenly Peace..."
 Action Comics #765
(Febuary, 2000): "A Clown Comes To Metropolis"
 Catwoman Vol. 1 #81 (April, 2000): "It's a Wonderful Life" [Note: Behind the Scenes]
 Joker/Mask #1 (of 4)
(May, 2000) [Note: Crossover w/ Dark Horse Comics]
 Catwoman Vol. 1 #82 (May, 2000): "Cat Outta Hell"
 Joker/Mask #2 (of 4)
(June, 2000) [Note: Crossover w/ Dark Horse Comics]
 Catwoman Vol. 1 #83 (June, 2000): "Scavenger Hunt"
 Joker/Mask #3 (of 4)
(July, 2000) [Note: Crossover w/ Dark Horse Comics]
 Catwoman Vol. 1 #84 (July, 2000): "The Lesser of Two Evils"
 Superman: Emperor Joker #1
(August, 2000): "It's a Joker's World, Baby, We Just Live In It!"
 Superman #161 (August, 2000): "You Say You Want A Revolution?"
 Adventures of Superman #583
(August, 2000): "Life Is But A (Very Bad) Dream"
 Superman: Man of Steel #105 (August, 2000): "All the World His Stage"
 Joker/Mask #4 (of 4)
(August, 2000) [Note: Crossover w/ Dark Horse Comics]
 Action Comics #770 (August, 2000): "He Who Laughs Last"
 Harley Quinn #1
(October, 2000): "An Harley Quinn Romance"
 Harley Quinn #2 (November, 2000): "A Heart Broken in Two"
 Wonder Woman Vol. 2 #164
(November, 2000): "Gods of Gotham, Part 1: Discord"
 Harley Quinn #3 (December, 2000): "Welcome to the Party!"
 Nightwing #52
(December, 2000): "Modern Romance" [Note: Cameo Appearence]
 Catwoman #89 (December, 2000): "Always Leave 'Em Laughing"
 Wonder Woman Vol. 2 #165
(December, 2000): "Gods of Gotham, Part Two: Avatars"
 Justice Leagues: Justice League of Amazons #1 (January, 2001): "Jungle Work"
 Birds of Prey #27
(January, 2001): "Officer Down, Part 3: Armed and Dangerous"
 Harley Quinn #4 (January, 2001): "The Wilde Life"
 Wonder Woman Vol. 2 #166
(January, 2001): "Gods of Gotham, Part Three: Fear"
 Harley Quinn #5 (Febuary, 2001): "Larger Than Life"
 Batman Gotham Knights #14
(Febuary, 2001): "The Bet"
 Wonder Woman #167 (Febuary, 2001): "Gods of Gotham, Part Four: Faith"
 Harley Quinn #6
(March, 2001): "Who Wants To Rob A Millionaire?"
 Harley Quinn #7 (April, 2001): "Gods And Mobsters"
 Harley Quinn #8
(May, 2001): "Be Cruel 2 Your School"
 Harley Quinn #9 (June, 2001): "Quintessence Part 1: Shop 'Til You're Dropped!"
 Harley Quinn #10
(July 2001): "Quintessence Part 2: Brilliant Mistake"
 Harley Quinn #11 (August 2001): "Quintessence Part 3: The Girl is Bats!"
 Harley Quinn: Our Worlds At War #1
(August 2001): "Our Fighting Forces"
 Harley Quinn #12 (September, 2001): "Quintessence Conclusion: A Date Which Will Live In Infamy!"
 Harley & Ivy: Love on the Lam
(September, 2001): "Love on the Lam"
 Joker: Last Laugh Secret Files #1 (October, 2001): "Touched", "Joker Fun Facts"
 Harley Quinn #13
(October, 2001): "Night and Day"
 The Joker: Last Laugh #3 (October, 2001): "Part Three: Lunatic Fringe"
 The Joker: Last Laugh #4
(October, 2001): "Part Four: Everyone Knows This Is Nowhere"
 The Joker: Last Laugh #5 (October, 2001): "Part Five: Mad, Mad World"
 Harley Quinn #14
(November, 2001): "Bright Lights, Big City Part 1: Welcome to Metropolis"
 Harley Quinn #15 (December, 2001): "Metropolis Mailbag"
 Harley Quinn #16
(January, 2002): "Both Sides Now"
 Adventures of Superman #600 (January, 2002): "Superman: The Dailies 2002, The Daily Planet" [Note: Back-up Story]
 Harley Quinn #17 (February, 2002): "#1 Am The Loneliest Number"
 Harley Quinn #18 (March, 2002): "The Bride of Bizarro!"
 Harley Quinn #19
(April, 2002): "Going Out With A Bang!"
 Harley Quinn #20 (May, 2002): "Wouldn't Be Caught Dead There"
 Harley Quinn #21
(June, 2002): "Hell and Highwater!"
 Batman Gotham Knights #30 (June, 2001): "Punchline"
 Harley Quinn #22
(July, 2002): "The Ballad of Harley Quinn"
 Harley Quinn #23 (August, 2002): "Men Are From Mars -- Woman Are From Hell!"
 Harley Quinn #24
(September, 2002): "I Ain't Got No Body--!"
 Harley Quinn #25 (October, 2002): "Once More, With Feeling!"
 Harley Quinn #26
(November, 2002): "Vengeance Unlimited, Part One"
 Harley Quinn #27 (December, 2002): "Vengeance Unlimited, Part Two"
 Harley Quinn #28
(January, 2003): "Vengeance Unlimited, Part Three"
 Harley Quinn #29 (February, 2003): "Vengeance Unlimited, Part Four"
 Batman #612
(February, 2003): "Hush, Chapter Five: The Battle" [Cameo]
 Harley Quinn #30 (March, 2003): "Vengeance Unlimited, Part Five"
 Batman #613
(March, 2003): "Hush, Chapter Six: The Opera"
 Harley Quinn #31 (April, 2003): "Who Steals From A Thief?"
 Batman #614
(April, 2003): "Hush, Chapter Seven: The Joke"
 Harley Quinn #32 (May, 2003): "The Year of the Rat"
 Batman #615
(May, 2003): "Hush, Chapter Eight: The Dead" [Hologram on Batcomputer]
 Harley Quinn #33 (June, 2003): "Behind Blue Eyes, Part One"
 Harley Quinn #34
(July, 2003): "Behind Blue Eyes, Part Two"
 Harley Quinn #35 (August, 2003): "Behind Blue Eyes, Part Three"
 Harley Quinn #36
(September, 2003): "Behind Blue Eyes, Part Four"
 Batman #619 (September, 2003): "Hush, Chapter Twelve: The End" ["Villains" Cover]
 Harley Quinn #37
(October, 2003): "Behind Blue Eyes, Part Five"
 Harley Quinn #38 (November, 2003): "Cuckoo For Incarceration"
 Detective Comics #789
(December, 2003): "The Tailor, Part 1" [Note: Costume Cameo]
 Infinite Crisis Special: Villains United #1 (April, 2005): "A Hero Dies But One " [Cameo]
 Infinite Crisis #7
(April, 2005): "Finale" [Note: Cover Only]
 Detective Comics #821 (July, 2006): "The Beautiful People" [Cameo]
 Detective Comics #823
(August, 2007): "Stalked" [Cameo]
 Batman #663 (March, 2007): "The Clown at Midnight"
 Detective Comics #831
(April, 2007): "Kind of Like Family"
 Birds of Prey #105 (April, 2007): "Whitewater, Part Two: Permafrost"
 Birds of Prey #106
(May, 2007): "Whitewater, Part Three: Storm Front"
 Birds of Prey #107 (June, 2007): "Whitewater, Part Four: A Heart Encased in Glass"
 Countdown #43
(July, 2007): "The Funeral"
 Countdown #42 (July, 2007): "Shock To The System"
 Batman Confidential #7
(July, 2007): "Lovers & Madmen, Part One: It's Funny"
 Catwoman #69 (July, 2007): "Catwoman Dies Conclusion" [Note: Cover Only]
 Birds of Prey #108
(July, 2007): "Whitewater, Epilogue: Swan Song"
 Countdown #41 (July, 2007): "Another Fine Mess"
 Countdown #40
(July, 2007): "Small Wonders"
 Countdown #39 (August, 2007): "Do Not Pass Go"
 Countdown #37
(August, 2007): "Forbidden Fruit" [Note: Also Has Cameo in Back-Up Story]
 Countdown #35 (August, 2007): "Girls Just Wanna Have Fun"
 Countdown #34
(September, 2007): "Searching For Answers"
 Countdown #31 (September, 2007): "New Frontiers"
 Countdown #30
(October, 2007): "Family Feud"
 Detective Comics #837 (October, 2007): "Honor Among Thieves"
 Countdown #29
(October, 2007): "Another Country Heard From"
Countdown #27 (October, 2007): "Disasters Great & Otherwise"
 Countdown to Final Crisis #21 (December, 2007): "Down and Out... And Beyond"
 Countdown to Final Crisis #19 (December, 2007): "Season's Beatings"
 Countdown to Final Crisis #18 (December, 2007): "Safe House" [Note: Earth 51 of Multiverse]
 Countdown to Final Crisis #16 (January, 2008): "What Price Paradise"
 Countdown to Final Crisis #15 (January, 2008): "Splitting the Atom"
 Countdown to Final Crisis #12 (February, 2008): "Come Together"
 Countdown to Final Crisis #11 (February, 2008)

Countdown to Final Crisis #10 (March, 2008) "The Gods Must Be Crazy" [Note: Also contains a two-page origin by Scott Beatty and Bruce Timm]


DC COMICS GRAPHIC NOVELS APPEARANCES

 Batman No Man's Land TP Vol. 4
(December, 2000)
 Batman No Man's Land TP Vol. 5 (March, 2001)
 Joker/Mask TP (April, 2001) [Note: Crossover w/ Dark Horse Comics]
 Wonder Woman: Gods of Gotham Collection
(May, 2001)
 Batman: Officer Down TP (August, 2001)
 Superman: 'Til Death Do Us Part
(December, 2001)
 Wonder Woman: Paradise Lost (January, 2002)
 Batman: Black and White Vol. 2
(August, 2002)
 Batman: Hush Vol. 2 (October, 2004)
 Superman: Emperor Joker
(December, 2006)
 Batman: Death in the City (November, 2007)
Harley Quinn: Preludes & Knock-Knock Jokes (January 2008)

BATMAN: THE ANIMATED SERIES APPEARANCES

Episode #022 - Joker's Favor
(September 11th, 1992)
Episode #033 - The Laughing Fish (January 10th, 1993)
Episode #046 - Almost Got 'Im (November 11th, 1992)
Episode #051 - The Man Who Killed Batman (February 01st, 1993)
Episode #056 - Harley and Ivy
(January 18th, 1993)
Episode #068 - The Trial (May 16th, 1994)
Episode #072 - Harlequinade
(May 23rd, 1994)
Episode #081 - Harley's Holiday (October 15th, 1994) [renamed The Adventures of Batman and Robin]
Episode #082 - Lockup
(November 19th, 1994)
Episode #086 - Holiday Knights (September 13th, 1997) [renamed The New Batman Adventures]
Episode #039 - World's Finest Part I
(October 4th, 1997) [Superman: The Animated Series]
Episode #040 - World's Finest Part II (October 4th, 1997) [Superman: The Animated Series]
Episode #041 - World's Finest Part III
(October 4th, 1997) [Superman: The Animated Series]
Episode #092 - Joker's Millions (February 21st, 1998)
Episode #097 - Over The Edge
(May 23rd, 1998)
Episode #105 - Girls' Night Out (October 17th, 1998)
Episode #106 - Mad Love
(January 16th, 1999)
Episode #108 - Beware The Creeper (November 7th, 1998)
Movie #001 - Return of the Joker
(October 30th, 2001) [Batman Beyond movie]
Episode #025 - Hard As Nails (January 25th, 2003) [Static Shock episode]
Episode #047 - Wild Cards, Part I
(December 7th, 2003) [Justice League episode]
Episode #048 - Wild Cards, Part II (December 7th, 2003) [Justice League episode]
Episode #047 - Two of a Kind
(February 24th, 2004) [The Batman episode]
Episode #050 - Rumors (March 3rd, 2004) [The Batman episode]
 
GOTHAM GIRLS APPEARANCES

Webisode #01 - The Vault
(July 27th, 2000)
Webisode #03 - Trick or Treat (August 24th, 2000)
Webisode #06 - More Than One Way (October 5th, 2000)
Webisode #08 - Three Belles (November 16th, 2000)
Webisode #09 - Gardener's Apprentice (November 30th, 2000)
Webisode #10 - Lady X
(December 14th, 2000)
Webisode #12 - Miss Un-Congeniality (June 19th, 2001)
Webisode #13 - Strategy
(July 3rd, 2001)
Webisode #14 - Baby Boom (July 17th, 2001)
Webisode #16 - Bat'ing Cleanup
(August 14th, 2001)
Webisode #17 - Cat Sitter (August 28, 2001)
Webisode #18 - Gotham Noir
(September 11th, 2001)
Webisode #19 - Scout's Dishonor (September 25, 2001)
Webisode #20 - I'm Badgirl
(July 16th, 2001)
Webisode #21 - Ms.-ing In Action (July 27th, 2002)
Webisode #22 - Gotham In Pink
(July 30th, 2002)
Webisode #23 - Hear Me Roar (August 13th, 2002)
Webisode #24 - Gotham In Blue
(August 27th, 2002)
Webisode #25 - A Cat In The Hand (September 10th, 2002)
Webisode #26 - Jailhouse Wreck
(September 24th, 2002)
Webisode #27 - Honor Among Thieves (October 8th, 2002)
Webisode #28 - No, I'm Batgirl!
(October 22nd, 2002)
Webisode #30 - Cold Hands, Cold Heart (November 19th, 2002)

BATMAN ADVENTURES APPEARANCES

 The Batman Adventures #12
(September, 1993): "Girl's Night Out"
 The Batman Adventures #28 (December, 1994): "Tweleve Days of Madness"
 The Batman Adventures Annual #1 (1994): "Laughter After Midnight"
 The Batman Adventures: Mad Love #1
(Febuary, 1994): "Mad Love"
 The Batman Adventures Holdiday Special #1 (January, 1995): "The Harley and the Ivy"
 Batman & Robin Adventures #1 (November, 1995): "Two-Timer"
 Batman & Robin Adventures #8 (July, 1996): "Harley and Ivy and... Robin?"
 Batman Chronicles Gallery #1 (1996) [Note: Pin-Up]
 Batman & Robin Adventures #18
(May, 1997): "Joker's Last Laugh!"
 Batgirl Adventures #1 (Febuary, 1998): "Oy to the World"
 Batman & Superman: World's Finest #1
(November, 1998): "World's Finest"
 Batman: Gotham Adventures #10 (March, 1999): "Mightier Than the Sword"
 Batman: Gotham Adventures #14
(July, 1999): "Masks of Love: A Harley Quinn Romance"
 Batman: Gotham Adventures #29 (October, 2000): "Six Hours To Kill"
 Batman: Gotham Adventures #30
(November, 2000): "Deals"
 Batman: Gotham Adventures #43 (December, 2001): "Turnabout"
 Batman: Gotham Adventures #45
(Febuary, 2002): "Running the Asylum"
 Batman Beyond: Return of the Joker Comic Adaptation (December, 2000)
 Batman: Gotham Adventures #51
(August, 2002): "Early Thaw"
 Gotham Girls #1 (October, 2002): "Cat's Paw"
 Batman: Gotham Adventures #53
(October, 2002): "Green Mind"
 Gotham Girls #2 (November, 2002): "Ivy League"
 Gotham Girls #3
(December, 2002): "Harlequinade"
 Justice League Adventures #13 (January, 2003): "Local Hero"
 Gotham Girls #4
(January, 2003): "I Carry A Badge"
 Gotham Girls #5 (February, 2003): "Bat Attitude"
 Batman: Gotham Adventures #58
(March, 2003): "Crazy Talk"
 Batman Adventures #1 (April, 2003): "No Asylum"
 Batman Adventures #3
(June, 2003): "My Boy-friend's Back", "Breathing Room"
 Batman Adventures #9 (December, 2003): "The Couch" [Note: Back-up Story]
 Batman Harley & Ivy #1
(April, 2004): "Bosom Buddies "
 Batman Harley & Ivy #2 (May, 2004): "Jungle Fever!"
 Batman Harley & Ivy #3
(June, 2004): "Hooray for Harleywood!"
 Batman Adventures #16 (July, 2004): "The Bride of the Joker"
 The Batman Strikes! #35
(July, 2007): "The Crown Prince of Late Night"
 The Batman Strikes! #39 (November, 2007) [Note: Cover Appearance]
 Justice League Unlimited #41 (March, 2008): "The Anniversary Party"
 The Batman Strikes #41 (January, 2008): "Girls Just Wanna Have Fun"

BIRDS OF PREY APPEARANCES

Episode 001 - Pilot
(October 9, 2002)
Episode 002 - Slick (October 16, 2002)
Episode 004 - Three Birds and a Baby (October 30, 2002)
Episode 006 - Primal Scream
(November 13, 2002)
Episode 007 - Split (November 20, 2002)
Episode 012 - Feat of Clay
(February 19, 2003)
Episode 013 - Devil's Eyes (February 19, 2003)

Harley's Haven is dedicated to the memory of Heath Ledger, an actor who devoted much to the role of The Joker.